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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Mol Immunol. 2015 Mar 28;66(2):208–215. doi: 10.1016/j.molimm.2015.03.008

Figure 4. Synergy among anti-CD40, CpG and MPL in Cytokine and chemokine production of PEC.

Figure 4

PEC were collected from naïve C57BL/6 mice or mice injected with anti-CD40 (0.5mg) three days earlier. Adherent cells were then cocultured in triplicate wells with B16 cells in the absence or presence of CpG (200ng/ml), MPL (20ng/ml) or their combination for 48 hours. Supernatants were tested for cytokines and chemokines including IL-12 (A), IFN-γ (B), MCP-1 (C), TNF (D), IL-6 (E) and IL-10 (F) using the CBA method. Data are represented as mean ± SE. A three-way ANOVA followed by Dunnett's post-testing was performed. *P<0.01; **P<0.001. The experiment was carried out in triplicate and repeated two times.