A. Skeletal muscle tissue (20 mg) from rats fed either AL or CR was homogenized
and expression of ceramidase, sphingosine kinase 1, sphingosine kinase 2 and SP
lyase examined by standard western blotting.
B. Quantitation of western blots. All proteins standardized by
β-actin.
C. Sphingosine quantities in 100 mg skeletal muscle tissue were quantified by
LC-MS/MS and standardized per mg of protein.
D. Sphingosine phosphate quantities in 100 mg muscle tissue were quantified by
LC-MS/MS and standardized per mg of protein.
Data are presented as mean ±SEM.
*P<0.05; n=14–15,
significant difference between the AL and CR groups according to a non-paired
students t-test.