Skip to main content
. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: J Nutr Biochem. 2015 Mar 5;26(7):687–695. doi: 10.1016/j.jnutbio.2015.01.007

Figure 6. Expression of proteins involved in ceramide catabolism and levels of the free sphingosine and sphingosine phosphate in skeletal muscle.

Figure 6

A. Skeletal muscle tissue (20 mg) from rats fed either AL or CR was homogenized and expression of ceramidase, sphingosine kinase 1, sphingosine kinase 2 and SP lyase examined by standard western blotting.

B. Quantitation of western blots. All proteins standardized by β-actin.

C. Sphingosine quantities in 100 mg skeletal muscle tissue were quantified by LC-MS/MS and standardized per mg of protein.

D. Sphingosine phosphate quantities in 100 mg muscle tissue were quantified by LC-MS/MS and standardized per mg of protein.

Data are presented as mean ±SEM. *P<0.05; n=14–15, significant difference between the AL and CR groups according to a non-paired students t-test.