Table 2.
MicroRNAs Differentially Expressed Among 50%, 80% and 100% BMSCs
| microRNAs | Confluence | P-Value | MSC activities | ||
|---|---|---|---|---|---|
| 50% | 80% | 100% | |||
| hsa-miR-17 | −3.13 | −3.41 | −4.18 | 2.9E-02 | Down-regulated in aging human MSC, targeting CDKN1A63 Accelerated 3T3L1 adipocyte differentiation by targeting Rb2/p13064 promote proliferation of USSC and prevent cell cycle arrest during neuronal lineage by targeting CCND1, E2F1, CDKN1A, PTEN, RB1, RBL1, RBL2, WEE165 |
| hsa-miR-20b | −2.50 | −3.10 | −4.22 | 4.4E-03 | up-regulated in ES-MSCs and targeting EPAS166 expressed in WJ-MSC and down-regulated during WJ-MSC neurogenesis67 |
| hsa-miR-106b | −2.40 | −2.61 | −3.37 | 1.1E-02 | Up-regulated in osteo-differentiated MSCs68 promote proliferation of USSC and prevent cell cycle arrest during neuronal lineage by targeting CCND1, E2F1, CDKN1A, PTEN, RB1, RBL1, RBL2, WEE165 |
| hsa-miR-29c | 0.57 | 0.08 | 0.20 | 1.7E-02 | Up-regulated in replicative senescence69, possibly targeting DNMT3A, DNMT3B69,70 increased during osteoblastic differentiation of murine cell line and involved in Wnt Signaling Pathway by targeting Sparc71 reduced expression of extracellular matrix genes72 |
| hsa-miR-138 | 0.92 | 0.71 | 0.14 | 1.4E-02 | Down-regulated during osteoblast differentiation of hMSCs; inhibited osteoblast differentiation of hMSCs in vitro and reduced ectopic bone formation in vivo by targeting FAK73 Down-regulated in osteo-differentiated MSCs68 Up-regulated in osteo-differentiated MSC49 Down-regulated during adipogenic differentiation of human ASC; inhibited adipogenesis by targeting EID-19 up-regulated during WJ-MSC neurogenesis67 |
| hsa-miR-26b | −0.39 | 0.82 | 0.49 | 4.3E-03 | up-regulated during osteoblast differentiation of hMSCs73 up-regulated during chondrogeneic differentiation of human BMSC, possibly targeting COL4A1, COL2A1, and COL6A174 up-regulated expression in wound tissue at the stage of active granulation formation75 Detected in human ES derived MSC76 |
| hsa-miR-130b | −0.62 | −0.07 | 0.08 | 2.1E-02 | Up-regulated in osteo-differentiated MSCs68 and chondro-differentiated MSCs74 Detected in human ES derived MSC76 up-regulated expression in wound tissue at the stage of active granulation formation75 |
| hsa-miR-494 | −0.23 | 0.20 | 0.59 | 4.9E-03 | Differentially expressed in osteo-differentiated MSC with Donor variance68 |
| hsa-miR-663 | −1.40 | 0.36 | 0.93 | 1.4E-04 | Differentially expressed in osteo-differentiated MSC with Donor variance68 Exclusively expressed in chondroblast derived from human MSC49 2 fold higher in MV than ES-MSC76 |
| hsa-miR-638 | −1.67 | 0.05 | 0.66 | 1.1E-05 | Exclusively expressed in chondroblast derived from human MSC49 down-regulated in osteo-differentiated MSC49 2 fold higher in MV than MSC76 |
| hsa-miR-486 | −0.37 | 0.20 | 0.59 | 2.8E-03 | Up-regulated in aging human ASCs, induces a premature senescence-like phenotype and inhibits proliferation; inhibits adipogenic and osteogenic differentiation by targeting SIRT177 |
USSC: unrestricted somatic stem cells from human cord blood. ES-MSC: human ES-derived MSC. WJ-MSC: Wharton's jelly matrix-derived mesenchymal stem cell. ASC: Adipose-tissue derived stem cell. MV: microvesicles