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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Mol Carcinog. 2014 Dec 9;55(1):3–14. doi: 10.1002/mc.22253

Figure 4.

Figure 4

The effect of silibinin and DHS on DNA binding activity of NF-κB and AP-1 in ASZ cells. ASZ cells were treated with DMSO, silibinin (100 µM), or DHS (30 µM) for 24 to 72 h and the DNA binding activity for NF-κB (A) and AP-1 (B) was determined by EMSA as detailed in ‘Materials and Methods’. (C) Supershift and competition assays were performed using nuclear extract from ASZ cells to confirm the specificity of NF-κB and AP-1 binding. The supershift assay for p50, p65, c-Fos, and c-Jun in ASZ cells is shown. Densitometry data presented below the bands are ‘fold change’ as compared with control (DMSO treated). ND: Not detectable.