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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Alcohol Clin Exp Res. 2014 Aug 26;38(9):2403–2413. doi: 10.1111/acer.12522

Figure 5. IAV-specific CD8 T cells from chronic ETOH mice are defective in their ability to kill IAV peptide-pulsed targets.

Figure 5

C57Bl/6 EtOH- and water-consuming mice were challenged with a sublethal dose of IAV. On day 8 p.i., the lungs of mice were harvested, homogenized, and CD8α+ cells were isolated by MACS. The effector T cells were then co-cultured with a 1:1 mixture of CFSEhi/IAV peptide-pulsed and CFSElo/OVA peptide-pulsed targets at either a 10:1 or 25:1 E:T ratio for 8 h. The percent of specific killing was determined by flow cytometry following incubation. n=5 mice/group. ** indicates p<0.01, *** indicates p<0.001