Table 2.
In vitro: Total EdU+ cells | |
---|---|
Treatment group | Total EdU+ cells |
0 Gy | 56.65 ± 2.03% |
0.5 Gy | 43.35 ± 3.71% |
8 Gy | 32.84 ± 3.39%* |
Note. SVZ = subventricular zone; NSPs = neural stem/progenitor cells; EdU = ethynyl deoxyuridine; ANOVA = analysis of variance. A single cell suspension of NSPs was exposed to γ rays at 0, 0.5, and 8 Gy. Prior to harvest, cells were incubated with 10 µM EdU overnight and then the cells were analyzed by flow cytometry and processed using Click-It chemistry for EdU. Data are averaged from three separate experiments. A *p < .05 by ANOVA followed by Dunnett’s multiple comparisons test compared with 0 Gy.