Table 6.
In vivo: Total EdU+ cells | ||||||||
---|---|---|---|---|---|---|---|---|
Treatment group | NSC | MP1 | MP2 | MP3/GRP2 | PFMP | BNAP/GRP1 | GRP3 | Other |
CD133+ | CD133− | CD133+ | CD133− | CD133− | CD133− | CD133− | ||
LeX+ | LeX+ | LeX+ | LeX− | LeX+ | LeX+ | LeX− | ||
NG2− | NG2− | NG2+ | NG2+ | NG2+ | NG2+ | NG2+ | ||
CD140a− | CD140a− | CD140a− | CD140a− | CD140a+ | CD140a− | CD140a+ | ||
EdU+ | EdU+ | EdU+ | EdU+ | EdU+ | EdU+ | EdU+ | ||
Nonirradiated control | 0.49% | 4.34% | 7.58% | 67.75% | 0.56% | 3.20% | 1.88% | 14.20% |
±0.17 | ±0.99 | ±1.69 | ±4.02 | ±0.01 | ±0.79 | ±0.20 | ±2.79 | |
Irradiated | 0.64% | 0.34%* | 3.65%* | 65.53% | 0.34% | 10.74%* | 6.14%* | 12.63% |
±0.10 | ±0.11 | ±0.82 | ±4.66 | ±0.06 | ±2.74 | ±1.17 | ±3.47 |
Note. MP = multipotential progenitor; PFMP = PDGF/FGF responsive multipotential progenitors; GRP = glial-restricted progenitor; BNAP = bipotential neuronal and astrocytic associated progenitor; NSPs = neural stem/progenitor cells; SVZs = subventricular zones; EdU = ethynyl deoxyuridine.
Mice were exposed to 0 or 8 Gy of γ rays. At 20 hr and 22 hr after irradiation, 100 mg/kg EdU was injected intraperitoneally. Two hours later the animals were euthanized and the SVZs were isolated, dissected, prepared, and analyzed with multicolor flow cytometry using CD133, LeX, CD140a, and NG2. They were processed using Click-It chemistry for EdU. Data are averaged from four animals per group. *p < .05 by unpaired t-test. Error bars indicate SEM.
p < .05 vs. nonirradiated control, n = 4.