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. 2015 Feb 18;21(1):1–14. doi: 10.2119/molmed.2015.00035

Figure 4.

Figure 4

Stimulatory effects of 3-MP on angiogenesis in vivo. (A–B) The effect of 3-MP on angiogenesis in vivo was assessed by Matrigel plug assay in C57BL/6 mice. Daily 3-MP treatments (0.3 and 1 mg/kg) significantly promoted neovascularization in the Matrigel plug assay. (A) Images of the Matrigel plugs immediately after collection at d 10 are shown. (B) Neovascularization in the Matrigel plugs was quantified by measuring hemoglobin content using Drabkin reagent, demonstrating the stimulatory effect of 3-MP on angiogenesis (*p < 0.05 versus vehicle; n = 5/group). (C–D) Following anesthesia and analgesia, rats were placed in a mold and subjected to burn injury by submerging the back in scalding (96–99°C) water for 10 s. Rats were randomly divided into two groups and treated daily for 28 d either with vehicle or 3-MP (300 μg/kg per day s.c.). Subcutaneous injections of 3-MP were performed daily at four equally spaced sites in the transition zone between burn site and healthy tissue. (C) Burn wound areas were determined on d 28 by NIS-Elements imaging software (Nikon) and (D) were quantified and analyzed statistically. 3-MP administration significantly reduced the wound area (*p < 0.05 versus vehicle; n = 8/group). In all bar graphs, mean ± SEM values are shown.