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. 2015 Jan 12;21(1):134–142. doi: 10.2119/molmed.2014.00189

Figure 6.

Figure 6

H5N1 HA is detected by TLR4 expressed on respiratory epithelial cells, facilitating the activation of JAK3, which leads to the impairment of cAMP-dependent CFTR channels. (A) Isc and ΔIsc measurements in HA-challenged tracheal tissues of JAK3+/+ mice with or without TLR4 inhibitor treatment (n = 5 per group, *p < 0.05 versus mice administered saline; #p < 0.05 versus mice pretreated with HA alone); (B) Immunofluorescent staining for phosphorylated JAK3 (p-JAK3) expression in 16HBE cells. Scale bars = 40 μm (C) Western blot for p-JAK3 in 16HBE cells. The mean optical densities of the bands for each group are presented (C, bottom) (*p < 0.05 versus saline control cells; #p < 0.05 versus cells incubated with HA alone for 60 min); c: control. (D) Quantitative RT-PCR analysis of TLR4 and CFTR gene expression in HA-treated calu-3 cells for the indicated periods compared with control.