Table 1. Breeding strategy used to generate TAPP1R211L/R211L, TAPP2R218L/R218L and TAPP1R211L/R211LTAPP2R218L/R218L knock-in mice.
Single homozygous TAPP1R211L/R211L and TAPP2R218L/R218L, as well as double TAPP1R211L/R211LTAPP2R218L/R218L, knock-in mice were bred using the strategy depicted in the Table and the progeny was genotyped as described in the Materials and methods section. The number and the percentage of each genotype are indicated followed by its expected Mendelian frequency. In the case of the double TAPP1R211L/R211LTAPP2R218L/R218L knock-in mice, as wild-type littermates and double knock-in mice can only be obtained at a ratio of 1:16 in the same cross, we interbred TAPP1R211L/R211LTAPP2R218L/R218L and TAPP1R211L/R211LTAPP2R218L/R218L as well as TAPP1+/+TAPP2+/+ and TAPP1+/+TAPP2+/+ littermates derived from a 1:16 ratio mating.
| Cross | Genotype | Number born | Expected Mendelian frequency (%) |
|---|---|---|---|
| TAPP1+/R211L and TAPP1+/R211L | TAPP1+/+ | 11 (24.45 %) | 25 |
| TAPP1+/R2111L | 23 (51.1 %) | 50 | |
| TAPP1R211L/R211L | 11 (24.45 %) | 25 | |
| TAPP2+/R218L and TAPP2+/R218L | TAPP2+/+ | 21 (31.8 %) | 25 |
| TAPP2+/R218L | 28 (42.4 %) | 50 | |
| TAPP2R218L/R218L | 17 (25.8 %) | 25 | |
| TAPP1+/R211LTAPP2+/R218L and TAPP1+/R211LTAPP2+/R218L | TAPP1+/+TAPP2+/+ | 6 (5.7 %) | 6.25 |
| TAPP1R211L/R211LTAPP2R218L/R218L | 5 (4.8 %) | 6.25 | |
| TAPP1+/R211LTAPP2+/R218L | 28 (26.7 %) | 25 | |
| TAPP1+/R211LTAPP2+/+ | 19 (18.1 %) | 12.5 | |
| TAPP1+/+TAPP2+/R218L | 14 (13.3 %) | 12.5 | |
| TAPP1R211L/R211LTAPP2+/+ | 4 (3.8 %) | 6.25 | |
| TAPP1+/+TAPP2R218L/R218L | 5 (4.8 %) | 6.25 | |
| TAPP1R211L/R211LTAPP2+/R218L | 14 (13.3 %) | 12.5 | |
| TAPP1+/R211LTAPP2R218L/R218L | 10 (9.5 %) | 12.5 | |
| TAPP1+/+TAPP2+/+ and TAPP1+/+TAPP2+/+ | TAPP1+/+TAPP2+/+ | 100% | |
| TAPP1R211L/R211LTAPP2R218L/R218L and TAPP1R211L/R211LTAPP2R218L/R218L | TAPP1R211L/R211LTAPP2R218L/R218L | 100% |