Figure 1.

DAT expression and function are increased in the dorsal striatum of the nRictor KO mouse. A, Representative immunoblots of surface and total protein fractions probed with a selective anti-DAT antibody or with an anti-Na-K ATPase antibody to serve as a loading control. TH, a cytosolic protein, was detected in the total fraction but not in the biotinylated fraction. Inset, Representative immunoblot of pAkt-473 in the dorsal striatum of nRictor KO mice and CTR animals. B, Quantitation of the optical density of DAT surface fraction normalized to CTR (n = 13). C, Quantitation of the optical density of the total protein fraction normalized to CTR (n = 16). D, [³H]-DA uptake in anatomically paired striatal slices obtained from CTR and nRictor KO mice expressed as a percentage of control (n = 18). *p < 0.05 (t test). **p < 0.01 (t test).