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. 2015 Jun 10;35(23):8843–8854. doi: 10.1523/JNEUROSCI.0887-15.2015

Figure 4.

Figure 4.

Elevated expression of D2R and not D1R increases pERK1/2 in the nRictor KO mice. A, Representative immunoblots (top) and optical density quantitation (bottom) of D2R expression in the dorsal striatum of nRictor KO and CTR animals. Actin was probed as a loading control. Data are expressed as percentage of CTR (n = 14). B, [3H]Nemonapride binding to striatal plasma membranes of CTR and nRictor KO mice: nonspecific binding (in the presence of 10 μm sulpiride) was subtracted from the specific binding, which was counted in duplicates in DPM and averaged within a sample. Data normalized to CTR levels (n = 8). C, Representative immunoblots (top) from striatal homogenates obtained from nRictor and CTR mice probed with D1R antibody, and quantitation of the respective optical densities (bottom). Data are shown as a percentage of control. Actin immunoblots served as a loading control (n = 12–13). D, Representative immunoblot and quantification of pERK1/2 (shown as a percentage of control) and total ERK in the dorsal striatum of CTR and nRictor KO. Actin is the loading control (n = 6–8). *p < 0.05 (t test). **p < 0.01 (t test).