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Expression of miR221 and miR222 in anti-RAGE pretreated CAL62 and BCPAP cells with or without HMGB1 for 24 h. CAL62 (a) and BCPAP cells (b) were treated with anti-RAGE antibody and then cultured with 10 nM HMGB1 for 24 h. Expression levels of mirR 221 and 222 were detected by RT-PCR and quantified with the ΔΔCt method. Histograms represent the mean values of 3 independent experiments (±SD).
