Table 1.
Primer sequences used to identify early and late chondrogenic markers.
| Target | Nucleotide sequence | Fragment length |
|---|---|---|
| Sox9 F | 5′-TTGAGCCTTAAAACGGTGCT-3′ | 244 bp |
| Sox9 R | 5′-CTGGTGTTCTGAGAGGCACA-3′ | |
|
| ||
| Col2a1 F | 5′-TCACGTACACTGCCCTGAAG-3′ | 213 bp |
| Col2a1R | 5′-TGCAACGGATTGTGTTGTTT-3′ | |
|
| ||
| CEP68 F | 5′-TCTTGTCCCATGGAGAGTCC-3′ | 154 bp |
| CEP68 R | 5′-GCCCCACTCTTCTTGGTGTA-3′ | |
|
| ||
| COMP F | 5′-CCATGGACTTGGCCAGTAGG-3′ | 385 bp |
| COMP R | 5′-GAGGAAGAGCCAGTGACGAAA-3′ | |
|
| ||
| TBP F | 5′-AGGCATCTGTCTTTGCACAC-3′ | 166 bp |
| TBP R | 5′-GGGTCAGTCCAGTGCCATAA-3′ | |
|
| ||
| YWHAZ F | 5′-AGGAGATAAAAAGAACATCCAGTCA-3′ | 269 bp |
| YWHAZ R | 5′-TATTCTCGAGCCATCTGCTGTTT-3′ | |