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. 2015 Jun 10;6:411. doi: 10.3389/fpls.2015.00411

Figure 4.

Figure 4

Agrobacterium-mediated genetic transformation of FEC of cassava cultivar TME14 and histochemical GUS assays of transformed and non-transformed tissues. (A) Agrobacterium-infected FEC proliferating on selective medium, (B) developing embryos on embryo induction medium, (C) maturing cotyledonary-stage embryos on shoot induction medium, (D) germination of cotyledonary-stage embryos on shoot induction and elongation medium, (E) transgenic plantlets regenerated on selective medium, (F) transient expression of gusA gene in FEC tissues 3 days after co-cultivation with Agrobacterium, (G) no expression of gusA gene in non-transgenic plant, (H) stable expression of gusA gene in transgenic plant.