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. 2015 Jun 10;6:411. doi: 10.3389/fpls.2015.00411

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Copyright © 2015 Nyaboga, Njiru and Tripathi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Molecular analysis of transgenic plants. (A) PCR analysis of putative transgenic lines of cassava using gusA and nptII gene specific primers. Lanes M; 1 kb plus molecular marker (Fermentas), P; pCAMBIA2301 plasmid control; NT, non-transgenic regenerated control plant; 1–10, transgenic lines regenerated, (B) RT-PCR analysis of transgenic cassava lines using gusA and PP2A gene specific primers. Amplified RT-PCR product designations were shown on the right, and products sizes were shown on the left. M, molecular weight marker; 1–9, different transgenic lines and NT, non-transgenic control plant.