Table 1.
Effect of Agrobacterium concentration, strains and co-centrifugation on transient GUS expression and number of cotyledonary stage embryos produced on selective medium.
| Factors | Treatments | Frequency of transient GUS expression (%) | Average no. of cotyledonary stage embryos on selective medium |
|---|---|---|---|
| Agrobacterium concentration | LBA4404 at OD600 of 0.1 | 25.59±4.17d | 59.67±4.16d |
| LBA4404 at OD600 of 0.25 | 63.63±4.73b | 131.33±8.02b | |
| LBA4404 at OD600 of 0.5 | 66.31±2.50b | 71.33±9.29c | |
| Agrobacterium strains | LBA4404 (OD600 of 0.25) | 65.38±4.12b | 137.45±7.32b |
| EHA105 (OD600 of 0.25) | 64.29±5.33b | 133.22±11.21b | |
| Co-centrifugation | Centrifugation (LBA4404, OD600 of 0.25) | 91.22±4.48a | 153.67±8.62a |
| No centrifugation (LBA4404, OD600 of 0.25) | 62.28±3.96b | 130.33±11.02b |
The values represent means ± SD, each from three independent experiments. Within each factor, means followed by different letters are significantly different (p ≤ 0.05) according to Duncan's multiple range test (DMRT).