. 2015 Jun 10;6:411. doi: 10.3389/fpls.2015.00411

Table 2.

Effect of BAP concentration, size of cotyledonary-stage embryos, silver nitrate and frequency of cotyledonary-stage embryos sub-culturing on efficiency of in vitro germination and regeneration of plantlets of cassava cultivar TME14.

Parameter tested	Treatment	Germination effeciency (%)	Mean no. of shoots per cotyledonary stage embryos
BAP concentration	BAP, 0.1 mg/l for 2 weeks followed by 0.4 mg/l for 2–4 weeks	70±6.67^b	2±1.00^b
	BAP, 0.4 mg/l for 2–6 weeks	40±3.33^d	1.33±0.58^c
	BAP, 0.1 mg/l for 2–6 weeks	41±4.27^d	1.03±0.35^c
Size of cotyledonary staged embryos	Entire cotyledonary staged embryos	68.89±6.94^b	2±1.00^b
	Longitudinally sliced cotyledonary staged embryos	36.67±6.67^e	1±0.00^c
	Transverse proximal half of cotyledonary staged embryos	46.67±3.33^c	1.33±0.57^c
Silver nitrate	CEM (No AgNO₃ added)	71.11±6.94^b	1.67±1.15^b
	CEM supplemented with 4 mg/l AgNO₃	67.78±8.39^b	2.67±0.58^a
Sub-culturing frequency	8 days	81.11±1.92^a	2±1.00^b
	14 days	68.89±3.85^b	1.67±0.57^b
	No sub-culturing till shoot formation	43.29±5.33^d	1.13±0.33^c

Each treatment contained 30 cotyledonary-stage embryos and experiments repeated three times and the data are means ± SD. Within each factor, means followed by different letters indicate significant differences at p ≤ 0.05 according to DMRT.