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. 2015 Jun 10;6:411. doi: 10.3389/fpls.2015.00411

Table 2.

Effect of BAP concentration, size of cotyledonary-stage embryos, silver nitrate and frequency of cotyledonary-stage embryos sub-culturing on efficiency of in vitro germination and regeneration of plantlets of cassava cultivar TME14.

Parameter tested Treatment Germination effeciency (%) Mean no. of shoots per cotyledonary stage embryos
BAP concentration BAP, 0.1 mg/l for 2 weeks followed by 0.4 mg/l for 2–4 weeks 70±6.67b 2±1.00b
BAP, 0.4 mg/l for 2–6 weeks 40±3.33d 1.33±0.58c
BAP, 0.1 mg/l for 2–6 weeks 41±4.27d 1.03±0.35c
Size of cotyledonary staged embryos Entire cotyledonary staged embryos 68.89±6.94b 2±1.00b
Longitudinally sliced cotyledonary staged embryos 36.67±6.67e 1±0.00c
Transverse proximal half of cotyledonary staged embryos 46.67±3.33c 1.33±0.57c
Silver nitrate CEM (No AgNO3 added) 71.11±6.94b 1.67±1.15b
CEM supplemented with 4 mg/l AgNO3 67.78±8.39b 2.67±0.58a
Sub-culturing frequency 8 days 81.11±1.92a 2±1.00b
14 days 68.89±3.85b 1.67±0.57b
No sub-culturing till shoot formation 43.29±5.33d 1.13±0.33c

Each treatment contained 30 cotyledonary-stage embryos and experiments repeated three times and the data are means ± SD. Within each factor, means followed by different letters indicate significant differences at p ≤ 0.05 according to DMRT.