Figure 3. ClpP knockout mice are viable with normal hematopoiesis.
(A) Mean ± SD hemoglobin (Hb), platelet (PLT), and white blood cell (WBC) counts from peripheral blood samples of WT and Clpp−/− mice (n = 4 per group).
(B) The frequency of Lin-cKit+Sca1+ hematopoietic cells in the bone marrow of WT and Clpp−/− mice. Mean ± SD percentages were determined by flow cytometry.
(C) Erythroid and myeloid colony formation from BFU-E and CFU-GM of hematopoietic cells from WT and Clpp−/− mice (n = 3 per group) plated in vitro under standard conditions. The mean ± SD colony counts are shown.
(D) The mean ± SD proportions of CD45.2 cells from WT or Clpp−/− mice transplanted in sublethally irradiated congenic W41/W41 CD45.1 mice measured by flow cytometry.
See also Figure S2.