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. Author manuscript; available in PMC: 2016 Apr 15.
Published in final edited form as: Vet Parasitol. 2015 Feb 7;209(0):1–42. doi: 10.1016/j.vetpar.2015.01.026

Figure 2.

Figure 2

Fluorescence images of S. neurona. (A) Images of gliding S. neurona merozoites stained with a monoclonal antibody (2A7-18) to the surface protein of Sn-SAG1. The formation of the trails is similar to those reported for Toxoplasma gondii. The trails are readily visualized by staining with antibodies to the major surface antigens. Gliding occurs on a variety of substrates, including coated chamber slides (50% PBS and fetal bovine serum). (B) Transgenic clone of Sarcocystis neurona expressing yellow fluorescent protein. Differential interference contrast image with epifluorescence image overlay showing a bovine turbinate cell monolayer containing a late-stage schizont and a mature schizont of a clone of S. neurona that stably expresses YFP. Host cell and parasite nuclei were stained with DAPI (blue). Bar= 10μm.