A) Metagene displaying the abundance of 5’P reads with respect to stop codons for 5PSeq of cells in rich media (in blue), after 5 or 30 minutes treatment with cycloheximide (in pink and magenta respectively) or a randomly fragmented sample (dotted light blue line) for S. pombe. Shown as in Figure 2.
B) Histograms showing the total number of reads in each reading frame within coding regions for 5PSeq YES (in blue), 5PSeq after cycloheximide treatment (in pink and magenta), and randomly fragmented sample (in light blue) for S. pombe.