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. 2015 Jun 10;5:11163. doi: 10.1038/srep11163

Figure 2. The pial basement membrane in the Pomgnt2-KO cortex begins to be disrupted at E11.5.

Figure 2

(a and b) Coronal sections of the forebrain from the control and Pomgnt2-KO embryos at E10.5 were immunostained with anti-laminin pAb and anti-CD31 pAb (a) and IIH6 mAb and anti-laminin pAb (b). (c) Coronal sections of the cerebral cortex from control and Pomgnt2-KO embryos at E11.5 were immunostained for laminin and CD31. Arrowheads indicate the emergence of the ectopic cell cluster at the gap in the breach of the pial basement membrane visualized by laminin immunostaining in the Pomgnt2-KO brain. (d) Enlarged images of the pial surface of the E11.5 Pomgnt2-KO brain in (c). Arrowheads indicate that laminin+/CD31– fragments derived from the pial basement membrane were observed over the ectopic cell cluster. The arrow points to the gap in the pial basement membrane. Scale bars represent 50 μm.