Figure 7. Reelin signaling is unchanged in the Pomgnt2-KO brain.

(a and b) Brain lysates prepared from control and Pomgnt2-KO embryos at E16.5 were analyzed by immunoblotting. Actin was used as a loading control. Total amounts of reelin and Dab1 were assessed using anti-reelin mAb (a) and anti-Dab1 pAb (b, input), respectively. The phosphorylation level of Dab1 was evaluated by immunoblotting with anti-phosphotyrosine (pTyr) mAb using samples immunoprecipitated with the anti-Dab1 antibody (b, IP: Dab1). IP, immunoprecipitation. The full-length blots with anti-reelin, anti-Dab1, and anti-pTyr antibodies are presented in Supplementary Figs. S1a and S1b.