Figure 7. Temporal changes of three different single neurons following our enzymatic digestion protocol.

Exposure time: (a) 0 min, (b) 30 min, (c) 1 hour. Of particular note is the morphological change occurring around the neuron such as the loss of plasma membrane integrity, thinner layers of satellite cells which are inhomogeneous, and increased staining intensity in the large nuclei of the treated sample (b and c). Nuclei of satellite cells distributed in the satellite cell region were also labeled by the DAPI stain. Scale bar is 100 μm.