Figure 5.
Prevention of H2O2-induced mitochondrial membrane depolarization by 15d-PGJ2. A-D: The JC-1 emission spectra between 520 nm to 620 nm were determined for cells under various conditions. (A): Untreated cells; (B): Cells treated with 1 μM 15d-PGJ2 overnight; (C): Cells treated with 1.5 mM H2O2 for 2 hours; (D): Cells treated with 1 μM 15d-PGJ2 overnight, then with 1.5 mM H2O2 (without 15d-PGJ2) for 2 hours. Note H2O2 caused a shift of the relative intensity of the peaks, and 15d-PGJ2 pre-treatment restored membrane potential to a condition closer to untreated cells. E-F: Cells were pretreated with 1 μM 15d-PGJ2 overnight, then with 1.5 mM H2O2 (without 15d-PGJ2) for 2 hours (E) or 4 hours (F), then the 545/595 emission intensity ratios were determined. Note in either 2-hour or 4-hour treatment, H2O2 caused an increase of the 545/595 emission intensity ratio, 15d-PGJ2 pre-treatment restored the ratio to that similar to control value (p < 0.001 between H2O2-treated and 15d-PGJ2+H2O2-treated cells in E and F).