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. 2015 May 17;13:45. doi: 10.1186/s12958-015-0033-0

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© Cederberg et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Proximal GSEs located at -179/-171 and -315/-310 of the porcine GnRHR promoter bind SF1. A, EMSAs performed with nuclear extracts and probes for the regions spanning either SF1 I or SF1 II. In addition, nuclear extracts were incubated with antiserum directed against SF1 (including its DNA binding domain) or normal rabbit IgG before the addition of radiolabeled probe (specific complex abrogation indicated by arrow). Electrophoresis of the SF1 I EMSA (left) was performed for an extended time, thus, free probe was run off the gel. B, Cells were transfected with -5118pGL3, μSF1IpGL3, μSF1IIpGL3 or pGL3. An asterisk indicates values that are greater than pGL3 (P < 0.05).