Table 1.

Primers used to generate reporter vectors ^a

Name	Sequence
-5118pGL3 F	5′-CAGACAATTAGATTCCAGGGC-3′
Promoter R	5′-TCCTTCCCCAACTGATGTAG-3′
-1810pGL3 F	5′-GTTATGTGGAAGAGCCGGTG-3′
-1716pGL3 F	5′-TGGCTTGCAGAAACCTAACC-3′
-1666pGL3 F	5′-AGGCACTAATCCAGTGTCTGC-3′
-1548pGL3 F	5′-TACTCCTCTTGATTTCTGACTC-3′
μSF1IpGL3 F	5′-AAGTACACAAAACAAGTTGCGGCCGCTCTTTCACATTAAATATA-3′
μSF1IIpGL3 F	5′-ACAAAATTAAGCTTCGAAATCGATTCTTCACCTAGGAAAAAT-3′
μRXRpGL3 F	5′-AAAAATGTTGTGAAAACCCTGCAGTCTGCTGAGGTACTACAG-3′
proximal OF	5′-GTTATGTGGAAGAGCCGGTG-3′
proximal OR	5′-CTTTATGTTTTTGGCGTCTTCC-3′
μSF1IIIpGL3 F	5′-GAGTTTTGGTTTGTTTTAGCGGCCGCTTAGCAAATGAACCCTAT-3′
μNF-κBpGL3 F	5′-GAAACCTAACCCCATATTGAATTCGAGAGCAATGGTTCAGTA-3′
μOCT1pGL3 F	5′-CAAATGAACCCTATGTGAGAGCTCTGGTGTTTTGGCTTGCAG-3′
distal OF	5′-CAGAGAATGCTATTGCTCTC-3′
distal OR	5′-GTGTAAGTGTTGGAACCACATC-3′

^aBlock replacement mutation reporter vectors were generated with outer forward (OF) or outer reverse (OR) primers as described in the Methods. The proximal OR resides in pGL3 3′ of the insert. Italicized bases indicate the new restriction enzyme digest site that replaced the transcription factor binding site in the native sequence.