Table 1.
M. avium subsp hominissuis spiked rabbit mucosa CTs of MycMavMtc PCR
| Mah spiked rabbit mucosa | ||||
|---|---|---|---|---|
| high | medium | low | ||
| Dilution | DNA Probe | 7.66×106 (CFU/g) | 7.66×104 (CFU/g) | 7.66×102 (CFU/g) |
| 1/1 | ITSdenak | UD | UD | UD |
| IS1311 | 31.77 | UD | UD | |
| IAC | UD | UD | UD | |
| 1/2 | ITSdenak | 43.56 | UD | UD |
| IS1311 | 28.5 | 36.66 | UD | |
| IAC | 35.1 | 41.19 | 36.00 | |
| 1/4 | ITSdenak | 39.7 | UD | UD |
| IS1311 | 28.85 | 34.50 | UD | |
| IAC | 32.62 | 33.99 | 33.63 | |
Mah: M. avium subps. hominissuis, ITSdenak: detects Mycobacterium genus, IS1311: detects Mycobacterium avium, IAC: internal amplification control, UD: undetermined no threshold-crossing fluorescence, devR detecting Mtb complex members was UD for all assayed conditions