Figure 4.

Western blot and densitometry analysis of proteins. Effect of methanol extract of wheatgrass on the levels of cyclin D1 and p53 in human laryngeal cancer cells. The cells were treated with methanol extract of wheatgrass at T1-100 µg/ml and T2-200 µg/ml doses for 48 h. Forty μg protein from each samples (control and treated) were electrophoresed and western blot was carried out using antibody against cyclin D1, p53 and β-actin was detected as a loading control. Values are expressed as mean ± standard deviation of three independent experiments considered at the P ≤ 0.05 levels