Figure 5.

Effects of aerial and root extracts on expression of apoptosis-related proteins. Both aerial (A) and root (B) extracts induced all caspases activation in lung cancer cells. 5 μmol/mL of reversible aldehyde inhibitors, were used to confirm that the observed fluorescence signal in cell population is due to the activity of caspase-like proteases. TNF-α (C) and IFN-γ (D) protein expression is significantly induced in the cells. The expression of TNF-α and IFN-γ in A549 cells following treatment for 72 h with aqueous aerial or root extracts was determined by ELISA. Each data value indicates the mean ± SEM of four independent experiments. ** P < 0.01 and *** P < 0.001, significantly different as compared to the control group; Student's t-test