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Lysates from A549 cells were incubated with 20 μg/mL aerial (Lane 2) or 40 μg/mL root (Lane 3) extracts were analyzed by Western blotting for caspase-3, TNF-α and IFN-γ protein expression levels. Aerial- or root-treated cells show cleaved (activated) caspase-3, whereas untreated (Lane 1) cells have no cleaved caspase-3. β-actin was used as internal control
