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. 2015 May 23;15:53. doi: 10.1186/s12935-015-0206-0

Fig. 1.

Fig. 1

Electropherogram of RT-PCR products. After reverse transcription of mRNA extracted from HeLa cells and HaCat cells, the mRNA were amplified by MY09/11 primer and the products were electrophoresed in sepharose gel. Lane 3 is DNA Marker, from top to bottom are 2000, 1000, 750, 500, 250 and 100 bp. Lane 1 is the amplification products lane of cDNA in HeLa cells. The HPV L1 transcription product strip at 450 bp shows that there is L1 genetic expression in HeLa cells. Lane 2 is the GAPDH internal control lane in HeLa cells and its strip is present at 258 bp. Lane 4 is the GAPDH internal control lane in HaCat cells and its strip is present at 258 bp. Lane 5 is the MY09/11 universal primer amplification compared lane in HaCat cells and there is no amplified strip in this lane