Skip to main content
. 2015 Jun 10;5:11028. doi: 10.1038/srep11028

Figure 1. IU1 treatment reduces PrPC levels in N2a58 cells.

Figure 1

(a) N2a58 cells were treated with DMSO or 100 μM IU1 for 24 or 48 h. Lysates were analyzed by immunoblotting with anti-PrP (SAF32) and anti-β-actin antibodies. (b) The graph shows the PrPC levels in N2a58 cells after treatment with DMSO or 100 μM IU1 for 48 h from at least three independent experiments. Asterisk indicates significant difference (*P < 0.05). Mean ± SD. (c) N2a58 cell lysates were deglycosylated with PNGase F followed by immunoblotting with anti-PrP (SAF83) antibody. (d) Quantification of deglycosylated PrP from at least three independent experiments was performed as described in (c). Asterisk indicates significant difference (*P < 0.05). Mean ± SD. (e) N2a58 cells were treated with DMSO or 100 μM IU1 for 48 h. PrPC (SAF32; green) and nuclei (blue) were visualized. Bars: 10 μm. (f) During 48 h of IU1 treatment and 12 h prior to harvest, N2a58 cells were treated with DMSO or MG132 (1, 2.5, 5 μM). Lysates were analyzed by immunoblotting with anti-PrP (SAF32) and anti-β-actin antibodies. Numbers below the gel indicate relative expression of PrPC normalized to β-actin.