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. 2015 Jun 10;5:11028. doi: 10.1038/srep11028

Figure 3. IU1 treatment reduces PrPSc levels in prion-infected cells.

Figure 3

(a) ScN2a58 cells were treated with DMSO or 100 μM IU1 for 24 or 48 h. Lysates were PK-digested followed by immunoblotting with anti-PrP (M20) antibody. (b) Quantification of PrPSc after IU1 treatment for 48 h from at least three independent experiments performed as described in (a). Asterisk indicates significant difference (***P < 0.0001). Mean ± SD. (c) N2a58-FK cells were treated with DMSO or 100 μM IU1 for 24 or 48 h. Lysates were PK-digested followed by immunoblotting with anti-PrP (M20) antibody. (d) Quantification of PrPSc after IU1 treatment for 48 h from at least three independent experiments performed as described in (c). Asterisk indicates significant difference (***P < 0.0001). Mean ± SD. (e) N2a58 or ScN2a58 cells were treated with DMSO or 100 μM IU1 for 48 h. PrPSc (mAb132; green) and nuclei (blue) were visualized. Bars: 10 μm.