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. 2015 Jun 10;5:132. doi: 10.3389/fonc.2015.00132

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Copyright © 2015 Bruinsma, Aprelia, Kool, Macurek, Lindqvist and Medema.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Plk1 activity is first seen in the nucleus. (A) Stills from a movie showing false color-coded CFP/YFP emission ratios. The stills show control-, BI 2536-, and MLN 8054-treated U2OS cells expressing the diffusible FRET-based biosensor for Plk1 activity while entering mitosis. BI 2536 was used at a concentration of 100 nM; MLN 8054 at a concentration of 1 μM. (B) Stills from a movie showing false color-coded CFP/YFP emission ratios of U2OS cells expressing the H2B-tagged FRET based biosensor were treated as in (A). (C) Quantification of CFP/YFP-ratio of cells shown in (A). Error bars represent the SD of 10 individual cells. (D) Quantification of CFP/YFP-ratio of cells shown in (B). Error bars represent the SD of 10 individual cells; *p < 0.0001.