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. 2015 Jun 10;6:270. doi: 10.3389/fimmu.2015.00270

Figure 2.

Figure 2

Effect of in vitro peptide stimulation on co-regulatory receptor expression by CMV-/EBV-specific CD8+ T cells in patients with CHC and healthy individuals. A comparison of the mean fluorescence intensities (MFI) of (A) PD-1, (B) Tim-3, and (C) 2B4 expressions on CMV-/EBV-specific CD8+ T cells in healthy individuals (n = 12 for CMV; n = 10 for EBV) and patients with CHC (n = 12 for CMV, n = 14 for EBV) revealed a stronger increase of expression in CHC patients upon peptide stimulation in vitro. Indicated are individual values of co-regulatory receptor expression ex vivo and upon culture in vitro. Red horizontal bars indicate mean values. The increase of expression was also revealed by comparing the fold increase of expression of (D) PD-1, (E) Tim-3, and (F) 2B4 upon in vitro peptide stimulation in relation to the ex vivo expression. p-Values are indicated. Cells were gated on lymphocytes and total CD8+ T cells after exclusion of dump channel.