Table 2.
List of RNA binding proteins associated with region 27 of the Lif 3′UTR
| Protein ID | Protein name | Gene name | 27 wt versus 27-No Significance | 27 wt versus 27-No Rel. levels | |
|---|---|---|---|---|---|
| Q15717 | ✩ | ELAV-like protein 1 | ELAVL1 | 1.88E-08 | 8.50 |
| P13693 | Translationally-controlled tumor protein | TPT1 | 0.0005 | 3.69 | |
| Q01469 | Fatty acid-binding protein, epidermal | FABP5 | 0.0007 | 3.58 | |
| Q12906 | ✩ | Interleukin enhancer-binding factor 3 | ILF3 | 0.0023 | 3.11 |
| P07741 | Adenine phosphoribosyltransferase | APRT | 0.0065 | 2.74 | |
| O75390 | Citrate synthase, mitochondrial | CS | 0.0069 | 2.72 | |
| Q9Y281 | Cofilin-2 | CFL2 | 0.0088 | 2.62 | |
| Q15185 | Prostaglandin E synthase 3 | PTGES3 | 0.0091 | 2.62 | |
| Q04760 | Lactoylglutathione lyase | GLO1 | 0.0095 | 2.60 | |
| A6NL28 | Putative tropomyosin alpha-3 chain-like protein | PTA3CLP | 0.0103 | 2.57 | |
| Q12905 | ✩ | Interleukin enhancer-binding factor 2 | ILF2 | 0.0119 | 2.52 |
| P61956 | Small ubiquitin-related modifier 2-3-4 | SUMO2-3-4 | 0.0131 | 2.48 | |
| P48735 | Isocitrate dehydrogenase [NADP], mitochondrial | IDH2 | 0.0150 | 2.43 | |
| Q96SI9 | ✩ | Spermatid perinuclear RNA-binding protein | STRBP | 0.0155 | 2.42 |
| P62633 | Cellular nucleic acid-binding protein | CNBP | 0.0173 | 2.38 | |
| P09104 | Gamma-enolase | ENO2 | 0.0200 | 2.33 | |
| P30086 | Phosphatidylethanolamine-binding protein 1 | PEBP1 | 0.0214 | 2.31 | |
| P20962 | Parathymosin | PTMS | 0.0219 | 2.30 | |
| P61326 | ✩ | Protein mago nashi homolog-B | MAGOH-B | 0.0225 | 2.29 |
| P50395 | Rab GDP dissociation inhibitor beta | GDI2 | 0.0233 | 2.28 | |
| Q14019 | Coactosin-like protein | COTL1 | 0.0234 | 2.27 | |
| P07737 | Profilin-1 | PFN1 | 0.0242 | 2.26 | |
| P00505 | Aspartate aminotransferase, mitochondrial | GOT2 | 0.0243 | 2.26 | |
| Q9BTT0 | Acidic leucine-rich nuclear phosphoprotein 32 family member E | ANP32E | 0.0248 | 2.25 | |
| O00299 | Chloride intracellular channel protein 1 | CLIC1 | 0.0268 | 2.22 | |
| Q9NR31 | GTP-binding protein SAR1a | SAR1A | 0.0280 | 2.21 | |
| Q8NBS9 | □ | Thioredoxin domain-containing protein 5 | TXNDC5 | 0.0289 | 2.20 |
| P29558 | ✩ | RNA-binding motif, single-stranded-interacting protein 1 | RBMS1 | 0.0311 | 2.17 |
| Q99471 | Prefoldin subunit 5 | PFDN5 | 0.0330 | 2.15 | |
| P13667 | □ | Protein disulfide-isomerase A4 | PDIA4 | 0.0336 | 2.14 |
| P60903 | Protein S100-A10 | S100A10 | 0.0365 | 2.11 | |
| P61088 | Ubiquitin-conjugating enzyme E2 N | UBE2N | 0.0381 | 2.10 | |
| P09382 | Galectin-1 | LGALS1 | 0.0410 | 2.07 | |
| P62834 | Ras-related protein Rap-1A | RAP1A | 0.0428 | 2.05 | |
| P09972 | Fructose-bisphosphate aldolase C | ALDOC | 0.0435 | 2.05 | |
| P30040 | Endoplasmic reticulum resident protein 29 | ERP29 | 0.0440 | 2.04 | |
| O60493 | Sorting nexin-3 | SNX3 | 0.0451 | 2.04 | |
| P22626 | ✩ | Heterogeneous nuclear ribonucleoproteins A2/B1 | HNRNPA2B1 | 0.0456 | 2.03 |
| Q15843 | NEDD8 | NEDD8 | 0.0464 | 2.03 | |
| P30101 | □ | Protein disulfide-isomerase A3 | PDIA3 | 0.0464 | 2.03 |
| P19338 | ✩ | Nucleolin | NCL | 0.0470 | 2.02 |
| O15511 | Actin-related protein 2/3 complex subunit 5 | ARPC5 | 0.0481 | 2.01 | |
| P00338 | L-lactate dehydrogenase A chain | LDHA | 0.0501 | 1.20 | |
| Q15121 | Astrocytic phosphoprotein PEA-15 | PEA15 | 0.0517 | 1.99 | |
| P62937 | Peptidyl-prolyl cis-trans isomerase A | PPIA | 0.0556 | 1.96 | |
| P14174 | Macrophage migration inhibitory factor | MIF | 0.0559 | 1.96 | |
| Q14103 | ✩ | Heterogeneous nuclear ribonucleoprotein D0 | HNRNPD | 0.0559 | 1.96 |
| Q99729 | ✩ | Heterogeneous nuclear ribonucleoprotein A/B | HNRNPAB | 0.0587 | 1.94 |
| O14979 | ✩ | Heterogeneous nuclear ribonucleoprotein D-like | HNRPDL | 0.0602 | 1.93 |
| P10599 | □ | Thioredoxin | TXN | 0.0602 | 1.93 |
RNAs of region 27 (‘R27 wt’) and its counterpart ARE-null region 27 (‘R27-No’) were used for RNA binding assays. Bound proteins were identified by quantitative mass spectrometry analysis and relative ratios were determined using total sequence reads for region ‘R27 wt’ and region ‘R27-No’. RNA binding proteins, (stars) and cell redox homeostasis proteins (squares) are indicated as identified by GO pathway analysis,(Additional file 6: File S1). The significance is the significance A P-value as determined by the Perseus software, defining the likelihood of a protein being enriched compared to the background distribution [112].