Fig. 4.

Growth and inhibition zones from bacterial cross streaks and on soft agar overlays. Representative A. wodanis strains positive for the bacteriocin locus (Aw⁺) were vertical streaked onto blood agar plates: A) A. wodanis 88/09/441, B) A. wodanis 06/09/139. Prependicular and in horizontal streaks: Mv1, M. viscosa 3632; Mv2, M. viscosa 06/09/139; Aw1⁺ (positive for the bacteriocin locus), A. wodanis 90/09/325; Aw2, A. wodanis 02/09/569. Reduced growth in the vicinity of Aw⁺ are illustrated for both M. viscosa (a) with Mv2 and for A. wodanis (b) with Aw2 where a clearly defined inhibition zone is observed. In the agar overlay method (c) and (D Aw1⁺ and Aw2 were first spotted onto the plates and grown to smal colonies before the agar overlay was added and bacterial suspensions of Mv2 (C) and Aw1⁺ (d) were plated on top. Colonies (Aw1⁺) surrounded by inhibition zones (marked with *) in the on-top bacterial lawn indicate bacteriocin activity