TABLE 1.

Summary of features and test principles of B19V-specific EIAs^a

Manufacturer	Antibody detected	B19V antigen(s)	Microwell coating	HRP conjugate	Total incubation time, temp
Biotrin	IgM	Baculovirus-expressed biotinylated VP2	Rabbit anti-human IgMb	Streptavidin	2 h 10 min, RT
Medac	IgM	Baculovirus-expressed VP1 and VP2	Mouse anti-human IgMb	Mouse anti-B19V Ig	3 h 30 min, RT
Mikrogen	IgM	E. coli-expressed VP1, baculovirus-expressed VP2	VP1 and VP2c	Anti-human IgM	2 h, RT and 37°C
Biotrin	IgG	Baculovirus-expressed VP2	VP2c	Rabbit anti-human IgG	1 h 40 min, RT
Medac	IgG	Baculovirus-expressed VP1 and VP2	VP1 and VP2c	Goat anti-human IgG	2 h 30 min, RT
Mikrogen	IgG	E. coli-expressed VP1, baculovirus-expressed VP2	VP1 and VP2c	Anti-human IgG	2 h, RT and 37°C

^aAbbreviations: HRP, horseradish-peroxidase; RT, room temperature.

^bMu capture EIA.

^cAntigen capture EIA.