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. 2004 Jul;42(7):3262–3271. doi: 10.1128/JCM.42.7.3262-3271.2004

FIG. 2.

FIG. 2.

Genus-, species-, and subtype-level microarray specificity. (A) Identical protozoan microarrays were individually hybridized with the labeled multiplex PCR products from the six protozoan strains shown in Fig. 1. The portions of the array dedicated to a particular genus are indicated on the left, whereas the original hybridization sample source is indicated above each array. The hybridization spots at the left and right ends of each array represent internal positive controls. (B) Microarray template showing the coordinates and identity of each probe (see Table 2) and the expected subarray hybridization pattern for each species, assemblage, or genotype. In each template, red spots represent E. histolytica, G. lamblia assemblage A, or C. parvum type 1-specific probes; yellow spots represent E. dispar, G. lamblia assemblage B, or C. parvum type 2-specific probes; and green spots represent probes common for both E. histolytica and E. dispar, G. lamblia assemblages A and B, or C. parvum types 1 and 2. (C) Observed subarray hybridization patterns.