TABLE 1.

Protozoan gene-targeted primers used in this study

Targeta	Name	Sequence (5′→3′)	Length (nt)	Tm (°C)b	GC (%)	PCR product size (nt)	Source or reference
Entamoeba
Locus1-2	R1	CTGGTTAGTATCTTCGCCTGT	21	50.6	48
	R2	CTTACACCCCCATTAACAAT	20	49.6	40	402/495	48
Locus5-6	R5A	CTAAAGCCCCCTTCTTCTATAATT	24	57.5	38
	R6A	CTCAGTCGGTAGAGCATGGT	20	49.6	55	485/510	48
ITS	18SP1	AGGTGAACCTGCGGAAGGATCATTA	25	62.6	48
	28SP2	TCATTCGCCATTACTTAAGAAATCATTGTT	30	65.2	30	433/423	34
sod-act	SAF2	GAGCTGCTTACTTAGAACATTGGTGG	26	59.1	46		14
	SAR2	CCAGATCCATTATCTACAACAAGTGC	26	57.0	42	497	This study
prrdx	prrdxF	GTCAAGAGAAAGAATGTTGTAAAGA	25	51.6	32		39
	prrdxR1	TTGATTTCTTTCAATTGTCCTGCA	24	56.3	33	274/304	This study
cpl	EntaCP1-F	GCAGCACTTGAAGGAAGATTATT	23	54.3	39
	EntaCP1-R	CCATCAACAACACCATATCCAA	22	52.2	41	441	This study
G. lamblia
hsp	ABB97F	AGGGCTCCGGCATAACTTTCC	21	60.5	57
	ABB220R	GTATCTGTGACCCGTCCGAG	20	51.5	60	163	32
tpiA	TPIAF	CGAGACAAGTGTTGAGATGC	20	47.7	50
	TPIAR	GGTCAAGAGCTTACAACACG	20	48.5	50	476	3
tpiB	TPIBF	GTTGCTCCCTCCTTTGTGCA	20	54.1	55
	TPIBR2	AGGCAATTACAACGTTCTCCCA	22	56.3	46	384	This study
giardin	GIA40F	CCGACGACCTCACCCGCAG	19	58.5	74		5
	GIA773R	GAGAGGCCGCCCTGGATC	18	56.3	72	749	This study
gdh	GDHF	CCGCTTCCACCCCTCTGTCAA	21	59.6	62		26
	GDHR	CCTTGCACATCTCCTCCAGGAA	21	57.0	55	389	This study
c4-orf	C4-F	AGCTCATCTTCGTCCTCTA	19	46.4	48		47
	C4-R	CAATCTTGTTTGCATACGA	19	46.2	37	445
C. parvum
cowp1	CpR1-F2	AAAGAAGCACCTCCTGTTTCAG	22	53.5	46		21
	CpR1-R2	GCAGCTGCTAATCTTCTTAGTGC	23	54.6	48	485
SSUrRNA	CpB-DIAGF	AAGCTCGTAGTTGGATTTCTG	21	50.8	43		30
	CpB-DIAGR	TAAGGTGCTGAAGGAGTAAGG	21	50.9	48	435
dhf	DHFR1	TTGTTGTGGCAGCTTCTGTTTTGA	24	57.7	42		30
	DHFR4	AAAGTTATCCTTTAAAGCATCCCT	24	56.8	33	359	This study
ptg	PolyTF1	TCCCAGTTCAAACTCACAAGAGTA	24	52.9	42		30
	PolyTR1	GGAGGAATAATACCACCATCTTCA	24	55.2	42	496	This study
RAPD	SB012F	CTCCGTTCGATGATGCAGATG	21	51.2	52		45
	SB012R	CGGCCCCTGTAGAAATAAGTCA	22	57.0	50	433
TRAP-C2	TRAP-C2F	CATATTCCCTGTCCCTTGAG	20	50.4	50		38
	TRAP-C2R	TGGACAACCCAAATGCAGAC	20	51.9	50	369
hsp	CPHSPT2F	TCCTCTGCCGTACAGGATCTCTTA	24	57.2	50
	CPHSPT2R	TGCTGCTCTTACCAGTACTCTTATCA	26	55.5	42	346	22
p23	P23-45	ATTATTTTTACGTTCCTTCCACTTG	25	57.0	32
	P23-569	AACCTTAATAAAAAACACTCTATTG	25	51.9	24	537	37

^aAbbreviations of target genes or gene products: ITS, intergenic sequence between rDNA; sod-act, intergenic region between superoxide dismutase and actin 3 genes; prrdx, perosiredoxin; cp1, cysteine protease 1; hsp, heat shock protein; gdh, glutamate dehydrogenase; tpi, triose phosphate isomerase; cowp, Cryptosporidium oocyst wall protein; ssu, small subunit; dhf, dihydrofolate reductase-thymidylate synthase; ptg, polythreonine-rich glycoprotein; TRAP-C2, thrombospondin-related anonymous protein 2.

^bBasic melting temperature (T_m) was calculated with Oligos software downloaded from (http://www.biocenter.helsinki.fi/bi/bare-1_html/oligos.htm).