TABLE 3.
Microarray versus PCR sensitivity with G. lamblia trophozoites
| Gene (no. of trophozoites) | Detection bya:
|
|||
|---|---|---|---|---|
| PCR
|
Microarray (no. positive/ no. of probes)
|
|||
| WB | GS-H7 | WB | GS-H7 | |
| hsp | ||||
| 200 | + | + | 2/2 | 2/2 |
| 5 | + | − | 2/2 | 2/2 |
| tpi | ||||
| 200 | + | + | 1/2 | 2/2 |
| 5 | − | + | 1/2 | 2/2 |
| giardin | ||||
| 200 | + | + | 6/6 | 6/6 |
| 5 | ? | ? | 6/6 | 0/6 |
| gdh | ||||
| 200 | + | + | 5/5 | 5/5 |
| 5 | − | − | 5/5 | 4/5 |
| c4 | ||||
| 200 | + | + | 4/4 | 4/4 |
| 5 | − | − | 4/4 | 4/4 |
a
Detection of each gene by PCR is designated as positive (+), ambiguous (?), or negative (−). Detection of each gene by microarray is represented by the number of positive spots out of the number of probes per gene.