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. 2014 Sep 29;105(10):1288–1298. doi: 10.1111/cas.12499

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© 2014 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Ectopic expression of miR-639 suppresses transforming growth factor beta (TGFβ)-induced epithelial-to-mesenchymal transition (EMT) in SCC9 and CAL27 cells. (a) miR-639 expression in the parental and TGFβ-treated tongue squamous cell carcinoma (TSCC) cells was determined using qRT-PCR. **P < 0.01 versus SCC9 or CAL27 cells. ##P < 0.01 versus mock transfection. (b) Western blotting and (c) immunofluorescence staining demonstrated that transfection with miR-639 mimics before TGFβ treatment increased E-cadherin (E-cad) expression and reduced the expression of vimentin (Vim) in SCC9 (TGFβ) and CAL27 (TGFβ) cells. β-actin was used as an internal control. Nuclei are shown in blue. Bar, 30 μm. (d) Modified Boyden chamber assays showed that the invasion and migration of SCC9 (TGFβ) and CAL27 (TGFβ) cells were suppressed by miR-639 mimics (original magnification, ×100).