Skip to main content
. 2014 Sep 18;105(10):1272–1278. doi: 10.1111/cas.12493

Figure 1.

Figure 1

Target sequence and structure of synthetic pyrrole–imidazole (PI) polyamide that targets the break fusion sites in TMPRSS2 and ERG. (a) PI poly-amide targeting break fusion sites. The polyamide was designed to bind to the break fusion site in TMPRSS2 and ERG. (b) Structure of the fusion polyamide and negative control polyamide. They were synthesized by employing a solid-phase method and purified by high performance liquid chromatography (0.1% AcOH/CH3CN, 0–66% linear gradient, 0–20 min, 254 nm, through a Chemcobond 5-ODS-H column). (c) Gel mobility shift assay and distribution of fluorescein isothiocyanate (FITC)-labeled PI polyamide in vitro. FITC-labeled DNA corresponding to the break fusion sites in TMPRSS2 was synthesized and incubated with vehicle (water), fusion PI polyamide, or negative control PI polyamide for 1 h at 37°C and loaded onto a 20% polyacrylamide gel.