Figure 6.
The fusion polyamide represses tumor formation of LNCaP cells in nude mice. (a, b) Seven-week-old male mice were implanted with 3 × 106 tumor cells and fusion polyamide or negative control polyamide was injected into the tail vein once weekly. (a) Photographs of mice bearing tumors after 4 weeks of treatment with pyrrole–imidazole (PI) polyamide. (b) Treatment with the fusion polyamide significantly reduced the tumor volume compared to treatment with the negative control polyamide. Line plots, means of tumor volume (V mm3) formed in mice; bars, SEM (n = 6 each), as determined by the formula: V = 0.5 × maximal diameter × middle diameter × minimal diameter. *P < 0.05 for fusion polyamide versus negative control polyamide. (c) Representative image of immunohistochemistry for cleaved caspase-3 in tumor xenograft tissues. (Magnification: ×400.)