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. 2014 Sep 29;105(10):1351–1359. doi: 10.1111/cas.12489

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© 2014 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Overexpression of guanine nucleotide-binding protein 1 (GBP1) in clinically relevant radioresistant (CRR) SAS, HepG2, KB, and MM102 cells. (a,b) Compared to corresponding parental cells, overexpression of GBP1 and ISG20 was found in all CRR cells. SAS cells had two different GBP1 target probes. (c) GBP1 but not ISG20 overexpression was reconfirmed in CRR cells by RT-PCR. In all the combinations of cell lines, CRR cells 12 h after 2-Gy irradiation (CRR+) showed higher GBP1 gene expression than parental cells without irradiation (P) and parental cells 12 h after 2-Gy irradiation (P+). (d) GBP1 protein expression in CRR cells and CRR-NOIR cells 12 h after 2-Gy irradiation or without irradiation were higher than their parental cells. NOIR cells were without irradiation for over 30 days, in order to avoid the influence of maintenance radiation. RNA from three independent exposure treatments was combined for the cDNA microarray and RT-PCR analysis. cDNA microarray analysis of the SAS group was carried out in duplicate.