Figure 5.

Distribution of guanine nucleotide-binding protein 1 (GBP1) in tumor tissues. (a) Compared with parental tumors, overexpression of the GBP1 gene and GBP1 protein was observed in clinically relevant radioresistant (CRR) tumor cells xenotransplanted into nude mice. (b) SAS-R1 tumors shrank due to fractionated radiation in combination with GBP1 suppression. SAS-R1 cells (1 × 10⁷) were injected into the right flank and SAS cells into the left flank of a mouse. (c) Representative positive staining of GBP1 in clinical cancer tissues. IHC, immunohistochemistry; IR, fractionated radiation with 2-Gy X-rays every 24h ISH, in situ hybridization; scr, scramble siRNA; arrows, GBP1 positive cells