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. 2014 Sep 25;105(10):1343–1350. doi: 10.1111/cas.12490

Figure 2.

Figure 2

Neutralization activity of CF172 against human CXC chemokine receptor-4 (CXCR4). The neutralization activity of CF172 for CXCR4 was measured using a cell-based assay. (a) Tango CXCR4-bla U2OS cells were incubated overnight at 37°C, followed by 5 h incubation in the presence of stromal cell-derived factor-1 (SDF1) (4 nM) and serially diluted CF172 (black square) or mIgG2b control (white triangle). Samples were then incubated for 2 h with LiveBLAzer FRET B/G substrate. Signals for 4 or 0 nM SDF1 with no antibody are indicated by the (+) or (−) dotted lines, respectively. (b) PathHunter CHO-K1 CXCR2 cells were incubated overnight at 37°C followed by 90 min incubation in the presence of CXCL8 (1.25 nM) and serially diluted CF172 (black square) or mIgG2b control (white triangle). Samples were then incubated for 1 h with PathHunter detection reagent. The signals for 1.25 or 0 nM CXCL8 with no antibody are indicated by the (+) or (−) dotted lines, respectively. All points indicate the mean ± SD (n = 3).