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. 2014 Sep 29;105(10):1360–1368. doi: 10.1111/cas.12495

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© 2014 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Immunohistochemical analysis of E2F in normal intermediate lobe of the pituitary (ILP) cells. (a) Normal ILP tissues from retinoblastoma gene (Rb1)^+/+ (left column), Rb1^D326V/+ (middle column) or Rb1^+/− (right column) mice were incubated with anti-E2F1 (upper row), anti-E2F2 (middle row) or anti-E2F3 (lower row) antibody. Bar, 50 μm. (b) The positive-cell ratio was analyzed in the ILP specimens treated with anti-E2F1 (upper panel), anti-E2F2 (middle panel) or anti-E2F3 (lower panel) antibody. The positive-cell ratios were calculated as described in Figure3(b). Statistical analysis: unpaired two-tailed Student's t-test. NS, not significant. *P < 0.05. **P < 0.01. Error bar, SD. n = 3 per genotype.