Fig 1.

Absence of promoter DNA hypermethylation of splicing factor genes in leukemic cell lines. Gene promoter maps show CpG sites (vertical black lines) and their distance from the transcriptional start site (+1) for each splicing factor gene. Recognition sites for respective restriction enzymes used in combined bisulfite restriction analysis (COBRA) assays are shown on the maps. For each splicing factor, the gel electrophoretogram of COBRA is shown below its respective gene promoter map. +ve Con, positive control, completely methylated human genomic DNA; N1, N2, N3, negative controls, peripheral blood DNA from three healthy individuals.